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sgrna/cas9n expression vector px335  (Addgene inc)


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    Structured Review

    Addgene inc sgrna/cas9n expression vector px335
    Sgrna/Cas9n Expression Vector Px335, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrna+cas9n+expression+vector/px330+u6+chimeric+bb+cbh+hspcas9/10__1172_slash_jci157431-294-12-16
    Average 90 stars, based on 1 article reviews
    sgrna/cas9n expression vector px335 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus
    Article Snippet: .. The guide RNAs were cloned into sgRNA/Cas9n expression vector (Addgene plasmid px335) as previously described . gE and TK homologous arms were amplified from PRV HNX strain by PCR. gEhm1-loxP-GFP-loxP-gEhm2 and TKhm1-loxN-mCherry-loxN-TKhm2 donor templates were constructed using overlapping PCR. ..

    Expressing:

    Article Title: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus
    Article Snippet: .. The guide RNAs were cloned into sgRNA/Cas9n expression vector (Addgene plasmid px335) as previously described . gE and TK homologous arms were amplified from PRV HNX strain by PCR. gEhm1-loxP-GFP-loxP-gEhm2 and TKhm1-loxN-mCherry-loxN-TKhm2 donor templates were constructed using overlapping PCR. ..

    Plasmid Preparation:

    Article Title: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus
    Article Snippet: .. The guide RNAs were cloned into sgRNA/Cas9n expression vector (Addgene plasmid px335) as previously described . gE and TK homologous arms were amplified from PRV HNX strain by PCR. gEhm1-loxP-GFP-loxP-gEhm2 and TKhm1-loxN-mCherry-loxN-TKhm2 donor templates were constructed using overlapping PCR. ..

    Amplification:

    Article Title: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus
    Article Snippet: .. The guide RNAs were cloned into sgRNA/Cas9n expression vector (Addgene plasmid px335) as previously described . gE and TK homologous arms were amplified from PRV HNX strain by PCR. gEhm1-loxP-GFP-loxP-gEhm2 and TKhm1-loxN-mCherry-loxN-TKhm2 donor templates were constructed using overlapping PCR. ..

    Polymerase Chain Reaction:

    Article Title: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus
    Article Snippet: .. The guide RNAs were cloned into sgRNA/Cas9n expression vector (Addgene plasmid px335) as previously described . gE and TK homologous arms were amplified from PRV HNX strain by PCR. gEhm1-loxP-GFP-loxP-gEhm2 and TKhm1-loxN-mCherry-loxN-TKhm2 donor templates were constructed using overlapping PCR. ..

    Construct:

    Article Title: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus
    Article Snippet: .. The guide RNAs were cloned into sgRNA/Cas9n expression vector (Addgene plasmid px335) as previously described . gE and TK homologous arms were amplified from PRV HNX strain by PCR. gEhm1-loxP-GFP-loxP-gEhm2 and TKhm1-loxN-mCherry-loxN-TKhm2 donor templates were constructed using overlapping PCR. ..



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